A couple of the comments were pings and I wouldn't have know how to get them back if I hadn't messed around with pinging for my WP_PingPreserver plugin, so I figured it might be useful to put up a quick bit on how to recover lost pings. You'll need to know a little bit about php to use it I guess but if you don't know php and need to restore some pings let me know and I'll make up a quick plugin. So here's some simple (i.e. probably not perfectly to specifications) php code to restore lost pings on Wordpress:

It's not very pretty but it gets the job done. Just replace the top three lines with the appropriate links, save it to your host and visit the file in your browser. Hopefully your host won't mysteriously lose your pings but if they do now you know what to do.

Welcome to the 104th edition of the Tangled Bank blog carnival (a biweekly showcase of good biology posts selected by the authors themselves). Rigorous calculations and archaelogical research have revealed that this is the Tangled Bank's 4th birthday. In the birthday spirit, several people sent appropriately themed presents.

Chris gave some great gifts (with a few caveats); a protien to resist radiation (may cause cancer), an enzyme to live longer, slimmer and stronger (but anti-socially and so far only in mice), and a transcription factor that can reverse skin aging (also in mice).

Even more microbiological gifts came in. steppen wolf wrapped up a nice box of cancer-fighting microRNA while Nimravid added some surprisingly robust bacterial gene networks. Finally, Ed chipped in a giant symbiotic bacteria with 40,000 copies of DNA and some influenza virus (straight from the flu's tropical Asian source) and I contributed some cancer fighting bacteria.

I'm running out of synonyms for "give" and ways to twist submissions into presents, so let's leave the birthday party behind and see the rest of the submissions.

First some plant related posts. Ocean Rambles has a bunch of nice pictures of the endangered Garry Oak ecosystem (and spring flowers) on Vancouver Island. Also concerned for plants, rENNISance woman links to the new idea of plant dignity (and a very odd stem cell comment thread).

On cultivated plants, Jeremy warns that relatively little money is being spent on farming research, especially for developing countries that need it most, and urges farmers to stop being pushed around by an agricultural corporation that sounds like the RIAA of farming (plus health effects).

Continuing the topic of corporate machinations, Biotunes describes an article (and personal experience) about bias in medical publications. On the lighter side of medicine, you can play doctor in space with a cool little flash game from the BBC.

Moving on to scheming of the creationist sort, Greg theorizes why physics doesn't have argumentum ad Nazium documentaries and points out that biology at the molecular scale is difficult to comprehend. Monado gives an example of this difficulty by comparing creationist drawings to a real electron micrograph of a flagellum. Late update: On the topic of pseudoscience, Podblack Cat asks "are women more superstitious?" (and throws in quite a literature review for the topic).

As an antidote to that intelligent design, Alvaro has details on making new neurons and a bunch of interviews of neuroscientists and cognitive psychologists.

Finally, 10,000 Birds (the only returning blog from Tangled Bank #1 [this post if you're curious]) describes coots (the bird, not the elderly).

I hope you enjoyed this Tangled Bank. The next edition is at the Beagle Project. You can email submissions to the hosts directly here or here or to the standard host@tangledbank.net before May 14th. Here's to four years of biology blogging carnivals and hopefully many more.

It looks like Gravatar has added Identicons and MonsterIDs to their system. I'd been wondering when they would add something like this since it seems like such a simple and useful addition. I was thinking my plugins wouldn't get much use now but it seems like they've gotten quite a few hits in the last few days. Not sure if that's related to Gravatar or just random but I guess some people are still finding them useful.

So I guess I'll keep maintaining the plugins since unfortunately Gravatar doesn't really give you all the configuration possibilities of WP_Identicon or the hand drawn monsters of WP_MonsterID.

Speaking of hand drawn, Lemm, the kind illustrator that drew up the new monsters, has (re?)started No More Tangerines, a blog showing off some of her art. It's pretty cool to see the progression her drawings go through.

I was doing a bit of background reading and came across an interesting paper about mutating normal bacteria into cancer-fighting bacteria. The paper centers around a single gene called inv (short for invasin) that can give an otherwise mild-mannered noninfectious bacteria the ability to invade cells.

Now this might seem like a pretty bad idea since there are probably enough infectious bacteria in the world already but this was only the first step of the research. Anderson and colleagues attached inv to a genetic switch (normally used for bacterial metabolism control) that turns on when arabinose (a type of sugar) is present. Unfortunately this switch was a little leaky. So even bacteria without arabinose were still infectious. Not ones to let that stop them, the researchers took out the ribosome (protein-making organelle) binding region of the gene, randomly mutated it and tested to find bacteria that were off by default but still able to turn on.

Once they got that working, they decided to attach a sensor to the infective gene. Bacteria often do things like switch metabolisms when they run out of oxygen. The researchers picked one of the bacteria genes that turns on when oxygen is low and replaced the arabinose switch from the previous bit with the oxygen sensing switch from this gene. Again the switch was leaky and they had to mutate it so it stayed off by default. Once that was done they had a bacteria that was only invasive in anaerobic environments. That's pretty cool because tumors are often anaerobic (since they're big lumps of fast growing dense tissue).

To go even further, the researchers tried to create bacteria that only turn on when there are many bacteria in one location. This will be useful because tumors often have higher concentrations of bacteria due to leaking nutrients and poor immune response. By creating a switch that only turns on when a bunch of bacteria are present, the bacteria can be further targeted to cancerous cells. To do this they used a gene from an ocean-dwelling bacteria that only turns on when many bacteria are present (the ocean bacteria uses the gene to detect when it has reached the light organ of squid). It seems odd that bacteria can communicate but it comes down to a simple mechanism made up of two genes. One gene encodes an enzyme that makes a chemical, called AI-1, that easily disperses in and out of the cell membrane. The second encodes a gene activator that is turned on by high concentrations of AI-1. When there are many bacteria, the environment becomes rich in AI-1 and the gene activator turns on even more production of AI-1 and gene activators. This positive feedback causes creates a sensitive switch that switches quickly from all off to all on when bacterial concentration crosses a certain level. By linking these genes to the infectious inv gene, the researchers created a bacteria that was only infectious when in high concentrations.

So now we have bacteria that might be able to selectively infect tumor cells. By combining this selective invasiveness with cell killing or immune response activating mechanisms, bacteria could become helpful tools for treating cancer (although there is still a pretty long way to go). The paper makes it look easy but that must have taken a good bit of work to get it all working so nicely. They ended up using DNA from three different bacteria species and many different bacterial systems. It's always really cool to see how scientists can take DNA "parts" and combine them together to create new and useful functions and even edit the DNA directly when the parts don't fit correctly.

I guess the next step in the research is to figure out how to get a bacteria to sense both an anaerobic and a high density environment. This might be a bit tricky since the two sensors would have to interact but I see some of the same researchers also have a paper on creating bacterial AND gates so I'll have to give that one a read too.

Reference

Anderson, J., Clarke, E., Arkin, A., Voigt, C. (2006). Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria. Journal of Molecular Biology, 355(4), 619-627. DOI: 10.1016/j.jmb.2005.10.076